Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA090775

Local Sample ID:	SC_20180803_RPLCp_FMS_Sup_S4_D1
Subject ID:	SU001312
Subject Type:	Bacteria
Subject Species:	Escherichia coli
Taxonomy ID:	562
Gender:	Not applicable

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Treatment:

Treatment ID:	TR001327
Treatment Summary:	For each isolate, a single colony from an agar dish was inoculated in 5 mL LB and shaken overnight at 37°C under ambient atmospheric conditions. Cultures were then diluted 1:1000 in the combined human urine and grown for 6 hours to mid-log phase (37°C, shaking), under 4% oxygen to emulate the bladder environment. After 6 hours, OD600 of each isolate was measured – and cultures were normalized by volume to yield equal number of organisms from each strain – prior to pooling into groups of 8 isolates each. CFUs were enumerated for each pool to confirm bacterial denisty (~109 total E. coli per pool). Each pool was then centrifuged to separate the cellular (pellet) and supernatant fraction. Pellets and supernatants were flash frozen and stored at -80°C until for metabolomic analysis.

Treatment ID:

TR001327

Treatment Summary:

For each isolate, a single colony from an agar dish was inoculated in 5 mL LB and shaken overnight at 37°C under ambient atmospheric conditions. Cultures were then diluted 1:1000 in the combined human urine and grown for 6 hours to mid-log phase (37°C, shaking), under 4% oxygen to emulate the bladder environment. After 6 hours, OD600 of each isolate was measured – and cultures were normalized by volume to yield equal number of organisms from each strain – prior to pooling into groups of 8 isolates each. CFUs were enumerated for each pool to confirm bacterial denisty (~109 total E. coli per pool). Each pool was then centrifuged to separate the cellular (pellet) and supernatant fraction. Pellets and supernatants were flash frozen and stored at -80°C until for metabolomic analysis.