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MB Sample ID: SA165041
| Local Sample ID: | 2h ATP_2 |
| Subject ID: | SU001856 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | Human Lung epithelial A549 cancer cells |
| Cell Passage Number: | 10 |
| Cell Counts: | 5 million cells |
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Treatment:
| Treatment ID: | TR001869 |
| Treatment Summary: | 5 million A549 cells were treated with control (no treatment), 0.5 mM ATP or 10 ng/ml TGF-beta for 2, 6 and 12 hours. |
| Treatment: | In vitro treatment with small molecules |
| Treatment Compound: | 0.5 mM ATP and 10 ng/ml TGF-beta |
| Treatment Dosevolume: | 10 ml DMEM media containing appropriate compounds |
| Cell Storage: | 37C; 5% CO2 incubator |
| Cell Growth Container: | 150 mm Dish tissue culture treated |
| Cell Media: | DMEM (10% FBS; 1% Pen/Strep) |
| Cell Harvesting: | after 2, 6 and 12 hours |
| Cell Pct Confluence: | 80% |
| Cell Media Lastchanged: | NA |
