Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA167621

Local Sample ID:	SAMP11_EV2
Subject ID:	SU001882
Subject Type:	Fungi
Subject Species:	Exidia glandulosa;Mucidula mucida
Taxonomy ID:	5219;139077

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Treatment:

Treatment ID:	TR001895
Treatment Summary:	F. sylvatica blocks (John Harrison, Wrexham, UK) with dimensions 2 cm3 were autoclaved three times and then pre-colonised by placing them on 0.5% malt-extract agar (MEA; 0.5% malt, 1.5% agar w/v; Sigma-Aldrich, Dorset, UK) cultures of the appropriate fungal strain and incubated in the dark at 20°C. Following 8-weeks pre-colonisation, experimental treatments consisting of 2 x 2 block matrices colonised with one of the two fungal strains per microcosm. Microcosms were subjected to a diurnally cycling temperature sequence of 5, 15, 25 and 15°C per 24 hours so that temperature changed by 10°C every 6 hours with a mean of 15 ± 10°C. Temperatures were chosen to represent the minima and maxima canopy-dwelling ligninolytic fungi encounter during the growing season in a temperate woodland ecosystem. Control microcosms were kept at a constant temperature of 15°C for the duration of the experiment. These methods were also chosen to be comparable with those of Toljander Lindahl, Holmer & Högberg (Toljander et al., 2006). All microcosms were incubated in the dark for 8 weeks with three replicates per species per treatment (n = 12).

Treatment ID:

TR001895

Treatment Summary:

F. sylvatica blocks (John Harrison, Wrexham, UK) with dimensions 2 cm3 were autoclaved three times and then pre-colonised by placing them on 0.5% malt-extract agar (MEA; 0.5% malt, 1.5% agar w/v; Sigma-Aldrich, Dorset, UK) cultures of the appropriate fungal strain and incubated in the dark at 20°C. Following 8-weeks pre-colonisation, experimental treatments consisting of 2 x 2 block matrices colonised with one of the two fungal strains per microcosm. Microcosms were subjected to a diurnally cycling temperature sequence of 5, 15, 25 and 15°C per 24 hours so that temperature changed by 10°C every 6 hours with a mean of 15 ± 10°C. Temperatures were chosen to represent the minima and maxima canopy-dwelling ligninolytic fungi encounter during the growing season in a temperate woodland ecosystem. Control microcosms were kept at a constant temperature of 15°C for the duration of the experiment. These methods were also chosen to be comparable with those of Toljander Lindahl, Holmer & Högberg (Toljander et al., 2006). All microcosms were incubated in the dark for 8 weeks with three replicates per species per treatment (n = 12).