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MB Sample ID: SA176875
Local Sample ID: | Ding_C-4_016 |
Subject ID: | SU001987 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | ATF3(+/+) vs ATF3(-/-) |
Cell Strain Details: | HCT116 cells engineered to knockout ATF3 expression via rAAV-mediated homologous recombination |
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Treatment:
Treatment ID: | TR001999 |
Treatment Summary: | HCT116 cells (~50% confluence) were washed with PBS, and cultured in HEPES buffered Krebs-Ringer solution supplemented with 25 mM [U-13C]-D-glucose, 10% dialysed FBS, 2×MEM amino acids, 2× Vitamin Solution and 4 mM L-glutamine, with or without 0.4 mM serine and glycine, for 24 h. Cells were washed with PBS and metabolites extracted using 1 mL of acetonitrile:isopropanol:water (3:3:2, v/v/v) mixture. |