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MB Sample ID: SA177831

Local Sample ID:	0060_LC_20190903_sample_0038
Subject ID:	SU001997
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Male and female

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Treatment:

Treatment ID:	TR002009
Treatment Summary:	Male and female, humanized PPARa mice (hPPARa) were generated from mouse PPARa-null, human PPARa-heterozygous breeding pairs (generously provided by Dr. Frank Gonzalez, NCI). At weaning, mice were provided a custom diet based on the What we eat in America (NHANES 2013/2014) analysis (Research Diets, New Brunswick, NJ) (USDA, 2018): 51.8% carbohydrate, 33.5% fat (soybean oil, lard and butter, with cholesterol at 224 mg/1884 kcal), and 14.7% protein, as a % energy intake. Fats are in the form of . Vehicle (VH) and treatment water were prepared from NERL High Purity water (23-249-589, Thermo Fisher Scientific), prepared with PFAS removal. Mice were administered vehicle (0.5% sucrose) drinking water or PFOA (8 mM +0.5% sucrose) drinking water ad libitum for 6-7 weeks. Food and water consumption were determined on a per cage basis each week and previously reported. Body weight was measured weekly. Aliquots of liver for lipidomics were flash frozen in liquid nitrogen and stored at -80?C. A total of 11 female mice (5 VH and 6 PFOA) and 12 male mice (7 VH and 5 PFOA) were analyzed.

Treatment ID:

TR002009

Treatment Summary:

Male and female, humanized PPARa mice (hPPARa) were generated from mouse PPARa-null, human PPARa-heterozygous breeding pairs (generously provided by Dr. Frank Gonzalez, NCI). At weaning, mice were provided a custom diet based on the What we eat in America (NHANES 2013/2014) analysis (Research Diets, New Brunswick, NJ) (USDA, 2018): 51.8% carbohydrate, 33.5% fat (soybean oil, lard and butter, with cholesterol at 224 mg/1884 kcal), and 14.7% protein, as a % energy intake. Fats are in the form of . Vehicle (VH) and treatment water were prepared from NERL High Purity water (23-249-589, Thermo Fisher Scientific), prepared with PFAS removal. Mice were administered vehicle (0.5% sucrose) drinking water or PFOA (8 mM +0.5% sucrose) drinking water ad libitum for 6-7 weeks. Food and water consumption were determined on a per cage basis each week and previously reported. Body weight was measured weekly. Aliquots of liver for lipidomics were flash frozen in liquid nitrogen and stored at -80?C. A total of 11 female mice (5 VH and 6 PFOA) and 12 male mice (7 VH and 5 PFOA) were analyzed.