Return to study ST001976 main page
MB Sample ID: SA185370
Local Sample ID: | mouse CSF 20 |
Subject ID: | SU002056 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Treatment:
Treatment ID: | TR002068 |
Treatment Summary: | Control AAV-GFP, MTX, AAV-SOD3 + buffers from Study Design. The pAAV-IRES-hrGFP vector was purchased from Agilent Technologies (240075) and pcDNA3.1-myc HisA (-)/ human SOD3 (myc-his tag at C-terminal) (36). Virus production and purification were performed by the Viral Core at BCH according to standard procedures (37). CD-1 mice (6-8 weeks) or Sprague Dawley rats (8 weeks) were given a single intravenous injection of 75 mg/kg MTX (Fisher Scientific, AAJ66364MD) dissolved in 0.9 % NaCl (Mountainside Medical, 0409-4888-10). MTX was freshly prepared for each experiment. MTX in mouse serum or CSF was assessed by MTX ELISA according to the manufacturer's instructions (Enzo Life Sciences, 142-0001). |