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MB Sample ID: SA185512
Local Sample ID: | pooled sample QC 1/10 dilution a |
Subject ID: | SU002059 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male and female |
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Treatment:
Treatment ID: | TR002071 |
Treatment Summary: | Mice were treated with PBS, PBS+ MTX, Null GFP with and without MTX, CAG-GFP with and without MTX, and CMV-GFP with and without MTX. buffers from study design were used for all respective samples. CD-1 mice (6-8 weeks) or Sprague Dawley rats (8 weeks) were given a single intravenous injection of 75 mg/kg MTX (Fisher Scientific, AAJ66364MD) dissolved in 0.9 % NaCl (Mountainside Medical, 0409-4888-10). MTX was freshly prepared for each experiment. MTX in mouse serum or CSF was assessed by MTX ELISA according to the manufacturer's instructions (Enzo Life Sciences, 142-0001). |