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MB Sample ID: SA186718

Local Sample ID:	QC_6
Subject ID:	SU002074
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Treatment
Sample Preparation
Chromatography
Analysis
MS

Treatment:

Treatment ID:	TR002086
Treatment Summary:	Cryopreserved plasma was processed for virus inactivation by adding 1500 µL of cold methanol:ethanol (MeOH:EtOH) in a 1:1 (v/v) proportion to 500 µL of plasma. Then, samples were vortex-mixed for 1 min, incubated on ice for 5 min and centrifuged at 16,000 x g for 20 min at 4 °C to precipitate and remove proteins. The clean upper layer or supernatant, which contained the metabolites of interest, was transferred to Eppendorf tubes and stored at -80 °C until analysis.

Treatment ID:

TR002086

Treatment Summary:

Cryopreserved plasma was processed for virus inactivation by adding 1500 µL of cold methanol:ethanol (MeOH:EtOH) in a 1:1 (v/v) proportion to 500 µL of plasma. Then, samples were vortex-mixed for 1 min, incubated on ice for 5 min and centrifuged at 16,000 x g for 20 min at 4 °C to precipitate and remove proteins. The clean upper layer or supernatant, which contained the metabolites of interest, was transferred to Eppendorf tubes and stored at -80 °C until analysis.