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MB Sample ID: SA192510

Local Sample ID:	repRGC_si_NPN
Subject ID:	SU002129
Subject Type:	Cultured cells
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116

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Treatment:

Treatment ID:	TR002141
Treatment Summary:	siRNA and overexpression. Cell transfection was carried using the INTERFERin transfection kit following manufacture’s recommendations (Polyplus Transfection, 89129-930). siRNA for LPCAT1, PLA2G4C, and LIPC were purchased from Ambion (Ambion, s102346, s107315, s67780 respectively), LPGAT1 and LIPC overexpression constructs were purchased from Genecopoeia (Genecopoeia, EX-mm15104-M61, EX-mm03119-M61). In brief, cells were transfected with 2 nM of siRNA or DNA in serum free cell culture media. INTERFERin reagent was added to the mix incubated at room temperature for 10 minutes and added to newly changed cell culture media for each well. Cells were incubated for 48 to 96 hours before harvesting.

Treatment ID:

TR002141

Treatment Summary:

siRNA and overexpression. Cell transfection was carried using the INTERFERin transfection kit following manufacture’s recommendations (Polyplus Transfection, 89129-930). siRNA for LPCAT1, PLA2G4C, and LIPC were purchased from Ambion (Ambion, s102346, s107315, s67780 respectively), LPGAT1 and LIPC overexpression constructs were purchased from Genecopoeia (Genecopoeia, EX-mm15104-M61, EX-mm03119-M61). In brief, cells were transfected with 2 nM of siRNA or DNA in serum free cell culture media. INTERFERin reagent was added to the mix incubated at room temperature for 10 minutes and added to newly changed cell culture media for each well. Cells were incubated for 48 to 96 hours before harvesting.