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MB Sample ID: SA194020
Local Sample ID: | KR4M2 |
Subject ID: | SU002143 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
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Treatment:
Treatment ID: | TR002155 |
Treatment Summary: | Method was described in previous studies (Jha et al., 2015; Lauterbach et al., 2019). 107 BMDMs per group were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs. |