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MB Sample ID: SA196398

Local Sample ID:	SRC-3 WT ZT0 replicate1 neg
Subject ID:	SU002162
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Male

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Treatment:

Treatment ID:	TR002174
Treatment Summary:	Mouse liver lipids were extracted using a modified Bligh-Dyer method. Briefly, 50 mg of crushed issue sample from mouse whole liver was used. A 2:2:2 volume ratio of water/methanol/dichloromethane was used for lipid extract at room temperature after spiking internal standards 17:0 LPC, 17:0PC, 17:0 PE, 17:0 PG, 17:0 ceramide, 17:0 SM, 17:0PS, 17:0PA, 17:0 TAG, 17:0MAG, DAG 16:0/18:1, CE 17:0. The organic layer was collected followed by a complete drying procedure under nitrogen.

Treatment ID:

TR002174

Treatment Summary:

Mouse liver lipids were extracted using a modified Bligh-Dyer method. Briefly, 50 mg of crushed issue sample from mouse whole liver was used. A 2:2:2 volume ratio of water/methanol/dichloromethane was used for lipid extract at room temperature after spiking internal standards 17:0 LPC, 17:0PC, 17:0 PE, 17:0 PG, 17:0 ceramide, 17:0 SM, 17:0PS, 17:0PA, 17:0 TAG, 17:0MAG, DAG 16:0/18:1, CE 17:0. The organic layer was collected followed by a complete drying procedure under nitrogen.