Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA212959

Local Sample ID:	109752
Subject ID:	SU002320
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Treatment:

Treatment ID:	TR002332
Treatment Summary:	U2-OS cells were seeded in 6 well plates. Etoposide treatment (1μM for 3 hours) was performed at different times to be able to terminate the experiment and extract the metabolites simultaneously for all samples. At the last timepoint – treatment for the no release samples – the medium was changed in all wells in order to have growth medium of the same composition at the time of metabolite extraction. Each sample was prepared in triplicates.

Treatment ID:

TR002332

Treatment Summary:

U2-OS cells were seeded in 6 well plates. Etoposide treatment (1μM for 3 hours) was performed at different times to be able to terminate the experiment and extract the metabolites simultaneously for all samples. At the last timepoint – treatment for the no release samples – the medium was changed in all wells in order to have growth medium of the same composition at the time of metabolite extraction. Each sample was prepared in triplicates.