Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA226680

Local Sample ID:	47
Subject ID:	SU002390
Subject Type:	Mammal
Subject Species:	Myotis lucifugus

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Treatment:

Treatment ID:	TR002402
Treatment Summary:	Bats were housed within mesh cages (Exo-terra Flexarium©; 22 cm x 35 cm x 43 cm) that were placed within temperature/humidity-controlled incubators (Caron© Environmental Chamber model 6041; 90.1 cm x 84.5 cm x 228.9 cm set to 8°C and 98% humidity) to encourage hibernation. Four cages were used to house the bats, separated by species and inoculation group; M. lucifugus (one sham-treatment: 16 individuals/cage, 10 male and 6 female; one Pd-inoculated; E. fuscus (8 individuals/cage, 4 male and 4 female). Bats were infected with a sham inoculum (20 μl, Phosphate Buffered Saline with Tween®20 (PBS-T)) or a Pd inoculum (20 μl, PBST containing 5x105 conidia) on wing and tail membranes. We monitored arousal frequency and sickness behavior using motion-activated infrared video. During the 71-day period bats found unresponsive or extremely sluggish were humanely euthanized (CO2 inhalation after isoflurane anesthesia and cervical dislocation), and were not included in this experiment. Several M. lucifugus showed signs of morbidity around day 55. We therefore, terminated the experiment 71 – 77 days post-inoculation (mid-March) which coincided with early stages of WNS progression. Disease severity was assessed by fungal load by qPCR33, UV fluorescence indicative of hyphae invasion, survival rate, and behavioral changes 32. E. fuscus was chosen as a WNS-resistant control and we found changes in their splenic lipidome to be minor. Tissue samples were collected, flash frozen, and shipped to Georgetown University Medical Center on dry ice and stored at -80°C until analysis.

Treatment ID:

TR002402

Treatment Summary:

Bats were housed within mesh cages (Exo-terra Flexarium©; 22 cm x 35 cm x 43 cm) that were placed within temperature/humidity-controlled incubators (Caron© Environmental Chamber model 6041; 90.1 cm x 84.5 cm x 228.9 cm set to 8°C and 98% humidity) to encourage hibernation. Four cages were used to house the bats, separated by species and inoculation group; M. lucifugus (one sham-treatment: 16 individuals/cage, 10 male and 6 female; one Pd-inoculated; E. fuscus (8 individuals/cage, 4 male and 4 female). Bats were infected with a sham inoculum (20 μl, Phosphate Buffered Saline with Tween®20 (PBS-T)) or a Pd inoculum (20 μl, PBST containing 5x105 conidia) on wing and tail membranes. We monitored arousal frequency and sickness behavior using motion-activated infrared video. During the 71-day period bats found unresponsive or extremely sluggish were humanely euthanized (CO2 inhalation after isoflurane anesthesia and cervical dislocation), and were not included in this experiment. Several M. lucifugus showed signs of morbidity around day 55. We therefore, terminated the experiment 71 – 77 days post-inoculation (mid-March) which coincided with early stages of WNS progression. Disease severity was assessed by fungal load by qPCR33, UV fluorescence indicative of hyphae invasion, survival rate, and behavioral changes 32. E. fuscus was chosen as a WNS-resistant control and we found changes in their splenic lipidome to be minor. Tissue samples were collected, flash frozen, and shipped to Georgetown University Medical Center on dry ice and stored at -80°C until analysis.