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MB Sample ID: SA245039

Local Sample ID:MG16_E4_2
Subject ID:SU002539
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Primary microglia isolated from APOE-targeted replacement mice homozygous for human E3 (B6.129P2-Apoe^tm2(APOE*3)Mae N8, Taconic #1548-F) or human E4 (B6.129P2- Apoe^tm3(APOE*4)Mae N8, Taconic #1549-F) alleles
Age Or Age Range:P0-P3
Gender:Pooled

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Treatment:

Treatment ID:TR002551
Treatment Summary:Cells were not treated, as we were just comparing APOE3/3 vs. APOE4/4 genotypes
Cell Growth Container:6-well plate
Cell Media:DMEM/F12 with 10% FBS, 10% LCCM supplement (see full methods in associated publication for preparation details), and 1% Penicillin/Streptomycin
Cell Envir Cond:37C, 5% CO2
Cell Harvesting:Cell scraper
Cell Pct Confluence:100
Cell Media Lastchanged:2-4 days prior to harvest
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