Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA249771

Local Sample ID:	MED1 Library 10 uM Aqueous Sample 2
Subject ID:	SU002593
Subject Type:	Synthetic sample

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Treatment:

Treatment ID:	TR002605
Treatment Summary:	Chemical libraries were added with individual molecules at a concentration of 100 nM, 1 uM or 10 uM to the condensate-forming low-complexity domain MED1 tagged with mCherry. Condensates were stimulated with 150 nM RNA. Condensates were centrifuged to the bottom of a 600 ul tube. Equal fractions from the input sample, aqueous phase and condensate phases were collected separately. Metabolites were extracted from each fraction.

Treatment ID:

TR002605

Treatment Summary:

Chemical libraries were added with individual molecules at a concentration of 100 nM, 1 uM or 10 uM to the condensate-forming low-complexity domain MED1 tagged with mCherry. Condensates were stimulated with 150 nM RNA. Condensates were centrifuged to the bottom of a 600 ul tube. Equal fractions from the input sample, aqueous phase and condensate phases were collected separately. Metabolites were extracted from each fraction.