Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA254516

Local Sample ID:	Hipp HPFM 1
Subject ID:	SU002624
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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MS

Treatment:

Treatment ID:	TR002636
Treatment Summary:	We designed a two-arm study, where euthanasia occurs through either 1) direct decapitation or 2) focused microwave (Fig. 1). In both arms, we performed rapid dissection of the brain followed by cryopreservation in liquid nitrogen. The major difference between the two arms is the tissue fixation or enzyme inactivation step occurs either during cryopreservation for decapitation (CP, ~90 seconds) or focused microwave (FM, ~0.6 second).

Treatment ID:

TR002636

Treatment Summary:

We designed a two-arm study, where euthanasia occurs through either 1) direct decapitation or 2) focused microwave (Fig. 1). In both arms, we performed rapid dissection of the brain followed by cryopreservation in liquid nitrogen. The major difference between the two arms is the tissue fixation or enzyme inactivation step occurs either during cryopreservation for decapitation (CP, ~90 seconds) or focused microwave (FM, ~0.6 second).