Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA254605

Local Sample ID:	T111
Subject ID:	SU002627
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR002639
Treatment Summary:	For genetically modified mice, LysM-Cre and Arg1fl/fl C57BL6J mice were bred to generate LysM-Cre +/+; Arg1fl/fl and litter mate control Arg1fl/fl mice. Orthotopic pancreatic cancer tumors were implanted in C57BL6J mice at 8-12 weeks of age. At endpoint, mice were euthanized by cervical dislocation, and tumors were harvested for TIF extraction. For Pharmacological inhibition, orthotopic tumors were implanted in C57BL6J mice at 8-12 weeks of age. 4 weeks after induction, once tumors were close end stage disease, CB-1158 (MedChem Express) dissolved in sterile water was administered by oral gavage at 100mg/kg. The acidity caused by the HCl in the drug solution was neutralized by adding equivalent amount of NaOH and an equivalent NaCl in sterile water solution was prepared as vehicle. 2hrs after treatment with CB-1158 or vehicle, mice were euthanized by cervical dislocation, and tumors were harvested for TIF extraction.

Treatment ID:

TR002639

Treatment Summary:

For genetically modified mice, LysM-Cre and Arg1fl/fl C57BL6J mice were bred to generate LysM-Cre +/+; Arg1fl/fl and litter mate control Arg1fl/fl mice. Orthotopic pancreatic cancer tumors were implanted in C57BL6J mice at 8-12 weeks of age. At endpoint, mice were euthanized by cervical dislocation, and tumors were harvested for TIF extraction. For Pharmacological inhibition, orthotopic tumors were implanted in C57BL6J mice at 8-12 weeks of age. 4 weeks after induction, once tumors were close end stage disease, CB-1158 (MedChem Express) dissolved in sterile water was administered by oral gavage at 100mg/kg. The acidity caused by the HCl in the drug solution was neutralized by adding equivalent amount of NaOH and an equivalent NaCl in sterile water solution was prepared as vehicle. 2hrs after treatment with CB-1158 or vehicle, mice were euthanized by cervical dislocation, and tumors were harvested for TIF extraction.