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MB Sample ID: SA305841
| Local Sample ID: | 8 |
| Subject ID: | SU002936 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
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Treatment:
| Treatment ID: | TR002945 |
| Treatment Summary: | The samples for this study were obtained from archived specimens from a cross-sectional survey that assessed the prevalence of VAD among a convenience sample of 207 lactating women in the province of Santa Margarita, Samar, Philippines. The original protocol was approved by the UC Davis IRB 290430-5 and the ethical committee of the local Ministry of Health in Eastern Visayas (Region VIII), Philippines. Excluded from the study were individuals who did not consent to further analysis of banked samples, had insufficient plasma remaining for metabolomics analysis, had samples that were not stored at the University of California, Davis, or had acute phase protein concentrations above normal range, including plasma C-reactive protein (CRP) > 5 mg/L or plasma α-1-acid glycoprotein (AGP) > 1.0 g/L (both measured by radial immunodiffusion). For the remaining samples eligible for metabolomics analysis, participants were divided into two groups with the lowest and highest concentrations based on their plasma VA concentrations. We selected 5 participants with plasma retinol ≤ 0.8 μmol/L and 5 participants with plasma retinol >1.05 μmol/L that were our low VA (VA-, < 0.8 μmol/L) or adequate VA (VA+, > 1.05 μmol/L) status groups, respectively. It must be noted that one participant in the VA- group had a plasma retinol concentration of 0.8 μmol/L, while the remaining four participants had plasma retinol ≤ 0.7 μmol/L, the cutoff for deficiency [8]. Casual breast milk retinol per gram of fat was also measured. Plasma samples analyzed in this study were collected from the antecubital vein in 7 mL Vacutainer® tubes containing K2-EDTA (Beckton Dickinson, Franklin Lakes, NJ, USA). Blood samples were shielded from light and placed in a cooler with ice packs prior to centrifugation to obtain plasma. Separated plasma samples were aliquoted into 2 ml cryovials and stored temporarily in a refrigerator until the end of data collection that day, and then frozen at -20⁰C for ~1-4 months, until transferred to Manila on dry ice, where they were stored first at -20 ºC and then later at -80ºC. Thereafter, samples were shipped on dry ice to the University of California, Davis and stored at -80ºC until analysis. |
| Treatment Protocol Filename: | La_Frano_Treatment_Protocol_v1[23].pdf |
