Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA313628

Local Sample ID:	placenta-WT-E18-C-02
Subject ID:	SU002983
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR002992
Treatment Summary:	Labeled glucose solution was prepared at a concentration of 100 mg/mL in filtered 0.9% sodium chloride solution. After overnight fasting (from 18:00 the day before), infusions took place around between 09:00 and 10:00 for all pregnant dams. Mice were anesthetized using isoflurane gas at 5% and placed on a warm pad. Mice were then kept under 2.5% isoflurane for the duration of infusion. For catheter placement, a 28-gauge insulin syringe needle was connected via polyethylene tubing (PE-10) to a syringe (containing glucose solution) placed on an infusion pump (Harvard Apparatus). At the start of the infusion, the 28-gauge needle was inserted into the tail vein. A glucose bolus of 4 µL/gBW was administered. Right after bolus administration, infusion rate was set at a continuous 0.085ul/gBW for a total infusion time of 3 hours.

Treatment ID:

TR002992

Treatment Summary:

Labeled glucose solution was prepared at a concentration of 100 mg/mL in filtered 0.9% sodium chloride solution. After overnight fasting (from 18:00 the day before), infusions took place around between 09:00 and 10:00 for all pregnant dams. Mice were anesthetized using isoflurane gas at 5% and placed on a warm pad. Mice were then kept under 2.5% isoflurane for the duration of infusion. For catheter placement, a 28-gauge insulin syringe needle was connected via polyethylene tubing (PE-10) to a syringe (containing glucose solution) placed on an infusion pump (Harvard Apparatus). At the start of the infusion, the 28-gauge needle was inserted into the tail vein. A glucose bolus of 4 µL/gBW was administered. Right after bolus administration, infusion rate was set at a continuous 0.085ul/gBW for a total infusion time of 3 hours.