Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA314642

Local Sample ID:	WT15-D3-P-02
Subject ID:	SU002991
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR003000
Treatment Summary:	Pregnant mice were placed under a maintained plane of isoflurane anesthesia (2.5%). Retro-orbital blood draw for time zero was performed followed by opening a small incision to the lower abdomen of the pregnant mouse to expose a single conceptus for fetal tissue harvesting. Immediately after the first blood draw and fetus collection, a tail vein catheter was placed and bolus (4 µL/gBW) was administered followed by continuous infusion (0.085ul/gBW/min). A single conceptus, accessed via the initial small incision, was harvested at multiple time points during infusion of the nutrient tracer (13C glucose). Retro-orbital blood draws (<20 ul) were taken throughout the infusion to monitor tracer enrichment in maternal blood. Timepoints for harvesting after time zero were: 30 minutes, 1 hour, 2 hours, 3 hours, and 4 hours.

Treatment ID:

TR003000

Treatment Summary:

Pregnant mice were placed under a maintained plane of isoflurane anesthesia (2.5%). Retro-orbital blood draw for time zero was performed followed by opening a small incision to the lower abdomen of the pregnant mouse to expose a single conceptus for fetal tissue harvesting. Immediately after the first blood draw and fetus collection, a tail vein catheter was placed and bolus (4 µL/gBW) was administered followed by continuous infusion (0.085ul/gBW/min). A single conceptus, accessed via the initial small incision, was harvested at multiple time points during infusion of the nutrient tracer (13C glucose). Retro-orbital blood draws (<20 ul) were taken throughout the infusion to monitor tracer enrichment in maternal blood. Timepoints for harvesting after time zero were: 30 minutes, 1 hour, 2 hours, 3 hours, and 4 hours.