Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA316761

Local Sample ID:	MINCH_Low_SN_-_2
Subject ID:	SU003032
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male

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Treatment:

Treatment ID:	TR003041
Treatment Summary:	SGSB preadipocytes were maintained at 37°C and 5% CO2 in 95% humidity. To examine the insulin response of SGBS treated with DINCH and MINCH, SGBS preadipocytes were treated for 18 days with differentiation media without the PPARG agonist rosiglitazone supplemented with DINCH or MINCH (10 nM and 10 µM). To obtain an adipogenesis reference, SGBS cells were differentiated with rosiglitazone for 18 days according to the standard differentiation protocol. After replacing the medium on day 17 with differentiation medium without insulin and overnight incubation (insulin starvation), cells were starved for 1 hour with differentiation medium without glucose and insulin (glucose + insulin starvation). Subsequently, cells were incubated with either differentiation medium with insulin (insulin-stimulated cells correspond to plus-insulin samples) or without insulin (unstimulated cells correspond to minus-insulin samples). During differentiation, a final concentration of 0.01% (v/v) MeOH and 0.02% (v/v) DMSO was added to all differentiation media. Continuous exposure was mimicked by replacing the cell culture medium every other day. Each treatment was performed in five biological replicates (n=5).

Treatment ID:

TR003041

Treatment Summary:

SGSB preadipocytes were maintained at 37°C and 5% CO2 in 95% humidity. To examine the insulin response of SGBS treated with DINCH and MINCH, SGBS preadipocytes were treated for 18 days with differentiation media without the PPARG agonist rosiglitazone supplemented with DINCH or MINCH (10 nM and 10 µM). To obtain an adipogenesis reference, SGBS cells were differentiated with rosiglitazone for 18 days according to the standard differentiation protocol. After replacing the medium on day 17 with differentiation medium without insulin and overnight incubation (insulin starvation), cells were starved for 1 hour with differentiation medium without glucose and insulin (glucose + insulin starvation). Subsequently, cells were incubated with either differentiation medium with insulin (insulin-stimulated cells correspond to plus-insulin samples) or without insulin (unstimulated cells correspond to minus-insulin samples). During differentiation, a final concentration of 0.01% (v/v) MeOH and 0.02% (v/v) DMSO was added to all differentiation media. Continuous exposure was mimicked by replacing the cell culture medium every other day. Each treatment was performed in five biological replicates (n=5).