Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA317366

Local Sample ID:	MINCH_10uM_4
Subject ID:	SU003035
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male

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Treatment:

Treatment ID:	TR003044
Treatment Summary:	SGSB preadipocytes were maintained at 37°C and 5% CO2 in 95% humidity. To investigate the effects of the plasticizer DINCH and its primary metabolite MINCH on adipocyte differentiation, SGBS preadipocytes were treated for 12 days with differentiation media without the PPARG agonist rosiglitazone supplemented with DINCH or MINCH (10 nM, 100 nM, 1 µM, and 10 µM). To obtain an adipogenesis reference, SGBS cells were differentiated in the presence of rosiglitazone; to obtain an untreated control, they were differentiated in the absence of rosiglitazone. A final concentration of 0.01% (v/v) MeOH and 0.02% (v/v) DMSO was added to all conditioned differentiation media. Continuous exposure was mimicked by replacing the cell culture medium every second day. Each treatment was performed in four biological replicates (n=4).

Treatment ID:

TR003044

Treatment Summary:

SGSB preadipocytes were maintained at 37°C and 5% CO2 in 95% humidity. To investigate the effects of the plasticizer DINCH and its primary metabolite MINCH on adipocyte differentiation, SGBS preadipocytes were treated for 12 days with differentiation media without the PPARG agonist rosiglitazone supplemented with DINCH or MINCH (10 nM, 100 nM, 1 µM, and 10 µM). To obtain an adipogenesis reference, SGBS cells were differentiated in the presence of rosiglitazone; to obtain an untreated control, they were differentiated in the absence of rosiglitazone. A final concentration of 0.01% (v/v) MeOH and 0.02% (v/v) DMSO was added to all conditioned differentiation media. Continuous exposure was mimicked by replacing the cell culture medium every second day. Each treatment was performed in four biological replicates (n=4).