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MB Sample ID: SA331537
| Local Sample ID: | T_1h_2 |
| Subject ID: | SU003168 |
| Subject Type: | Bacteria |
| Subject Species: | Staphylococcus aureus |
| Taxonomy ID: | 1280 |
| Genotype Strain: | ATCC 43300 |
| Age Or Age Range: | NA |
| Weight Or Weight Range: | NA |
| Height Or Height Range: | NA |
| Gender: | Not applicable |
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Treatment:
| Treatment ID: | TR003177 |
| Treatment Summary: | An untargeted metabolomics study was carried out to explore the mechanism(s) of action of C12-o-(BG-D)-10 against MRSA ATCC 43300 using a concentration of 48 µg/mL (i.e. 3×MIC). Samples were taken and analyzed at the 1-, 3-, and 6-h time points in 4 biological replicates. An overnight culture was prepared by inoculating a single colony into 100 mL CAMHB in 250 mL conical flasks (Pyrex) and incubating the suspension in a shaker at 37°C and 180 rpm for ~16 h. After incubation overnight, log-phase cells were prepared in fresh MHB and then incubated for 2 h at 37°C at 180 rpm to log phase with a starting bacterial inoculum of 108 CFU/mL. Then, C12-o-(BG-D)-10 was added to obtain the desired concentration of 48 µg/mL (3 x MIC), in parallel to a CLO-free control for each replicate. The flasks were then incubated at 37°C with shaking at 180 rpm. At each time point (0, 1, 3, and 6 h), 15-mL samples were transferred to 50-mL Falcon tubes for quenching, and the optical density reading at 600 nm (OD600) was then measured and normalized to the pre-treatment level of approximately ~0.5 with fresh CAMHB. Samples were then centrifuged at 3,220 g and 4°C for 10 min, and the supernatants were removed. The pellets were stored at -80°C until metabolite extraction. The experiment was performed in 4 biological replicates to reduce the bias from inherent random variation. |
| Treatment Dose: | 48 ug/ml |
| Cell Media: | CAMHB |
