Summary of Study ST001211
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000814. The data can be accessed directly via it's Project DOI: 10.21228/M8G40M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001211 |
Study Title | Metabolomic Markers of Methotrexate Response, In Vitro |
Study Summary | Erythroblastoid cells (K562) maintained in logarithmic growth phase were treated with 1000 nM methotrexate or vehicle alone (i.e. D-PBS) under standard culture conditions for 24 hours. Cellular response to methotrexate was measured based on anti-proliferative activity by cell counting. Cells were washed, flash frozen in liquid nitrogen and submitted for metabolomics analysis to the NIH West Coast Metabolomics Center. |
Institute | University of Kansas |
Department | Pharmacy Practice |
Laboratory | Funk Lab |
Last Name | Funk |
First Name | Ryan |
Address | 2106 Olathe Boulevard |
ryanfunk@kumc.edu | |
Phone | 9135885000 |
Submit Date | 2019-07-03 |
Num Groups | 2 |
Total Subjects | 20 samples |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2020-01-06 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001293 |
Treatment Summary: | Cells were maintained under normal growth conditions in a humidified, temperature- and CO2-controlled incubator at 37ºC and 5% CO2. Cells were kept at density between 2.0x105 and 1.0x106 cells/mL to maintain logarithmic growth conditions to promote maximum proliferative activity over the experimental period. All experiments were conducted within six passages following removal from cryopreservation. On the day of experimentation, K562 cells were seeded at a density of 4.0x105 cells/mL and exposed to 1000 nM MTX or D-PBS (i.e. vehicle) for 24 hours under normal growth conditions. |
Treatment Compound: | Methotrexate |
Treatment Route: | In Media |
Treatment Dose: | 1000 nM |
Treatment Doseduration: | 24 hours |
Treatment Vehicle: | Dulbecco's Phosphate Buffered Saline (without Calcium/Magnesium) |
Cell Storage: | Liquid Nitrogen |
Cell Growth Container: | T-175 Flask |
Cell Growth Config: | Suspension |
Cell Inoc Proc: | Seeded at 4.010^5 cells/mL |
Cell Media: | RPMI 1640 medium (catalog no. 61870-127, ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (catalog no. S11150, Atlanta Biologicals, Lawrenceville, GA) along with 100 units/mL penicillin and 1,000 units/mL streptomycin (catalog no. 15140122, ThermoFisher Scientific, Waltham, MA) |
Cell Envir Cond: | humidified, temperature- and CO2-controlled incubator at 37ºC and 5% CO2 |
Cell Harvesting: | Counted and a volume containing 15x10^6 cells were collected, washed, flash frozen in liquid nitrogen |
Cell Pct Confluence: | NA |
Cell Media Lastchanged: | 24 hours prior to collection |