Summary of Study ST003150
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001958. The data can be accessed directly via it's Project DOI: 10.21228/M8MX5P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003150 |
Study Title | Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on gut microbiota and host glucose homeostasis in C57BL/6J male mice (Part II) |
Study Summary | This study aimed to explore the association between early-life exposure to a potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the microbiota, leading to impaired metabolic homeostasis later in life. This study utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and biochemical assays to analyze the gut microbiome composition and function, as well as the physiological and metabolic effects of early-life exposure to 2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in vitro was assessed using optical density (OD 600), flow cytometry, transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice exhibited disruption in the gut microbiome community structure and function, characterized by lower abundances of A. muciniphila, lower levels of cecal short chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes associated with early-life POP exposure were transferable to GF recipients in the absence of POP carry-over. In addition, AHR-independent interactions between POPs and the microbiota were observed, significantly affected the growth, physiology, gene expression, and metabolic activity of A. muciniphila, resulting in suppressed activity along the ILA pathway. |
Institute | Pennsylvania State University |
Department | Department of Veterinary and Biomedical Sciences |
Last Name | Koo |
First Name | Imhoi |
Address | 307B Life Science Building |
iuk41@psu.edu | |
Phone | 8148107425 |
Submit Date | 2024-03-28 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2024-04-30 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
Treatment ID: | TR003276 |
Treatment Summary: | 1. After feed training, mice were fed pills containing TCDF (dissolved in acetone) or acetone alone as vehicle, and one pill uniformly contained 0.46 µg TCDF (24 µg/kg as final dose). Mice were housed singly in an empty cage and monitored to ensure the pill was consumed in the morning for 5 days. 2. TCDF (24 µg/kg) or corn oil as vehicle were administered to age-matched male GF and Ahr-/- mice by oral gavage once daily for five days (n = 4 per group). 3. Four-week-old male GF C57BL/6J mice were orally gavaged with 100 µL of cecal suspension (100 mg in 1 mL sterile BHI CHV media) from vehicle or TCDF treated mice in long-duration model. 4. A. muciniphila was administered to GF mice by oral gavage at one dose 107 CFU/0.1 mL suspended in sterile BHI CHV media containing an end concentration of glycerol (15% vol/vol). |