Summary of Study ST001987

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001263. The data can be accessed directly via it's Project DOI: 10.21228/M8FX3C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001987
Study Title	Transcriptomic and lipidomic analysis unravels the response of Faecalibacterium prausnitzii to calcium palmitate
Study Summary	Infant formula is a suggested alternative to human milk if breastfeeding is not an option; vegetable oil blends are commonly used in infant formula (IF) to replace dairy fat, which can induce the formation of the poorly soluble soap calcium palmitate (CP) in the infant’s gut. Previously, we observed that CP at a low concentration of 0.01 mg/ml inhibits the growth of dominant infant bacteria such as Faecalibacterium prausnitzii both during the exponential phase as well as in the stationary phase. Here, we investigate the underlying mechanism of the CP inhibition on infant-gut bacteria using F. prausnitzii as a model by analysing its growth at a transcriptomic and lipidomic level.
Institute	University of Groningen
Last Name	Horvatovich
First Name	Péter
Address	Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
Email	p.l.horvatovich@rug.nl
Phone	+31 (0)50 363 3341
Submit Date	2021-11-12
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2021-12-15
Release Version	1

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Treatment:

Treatment ID:	TR002080
Treatment Summary:	The diluted lipid solution was separated using a Zorbax Eclipse Plus C18 column (1.8 μm, 50 × 2.1 mm) on an Acquity UPLC system (Waters, Manchester, UK). Mobile phases consisted of 10 mM ammonium formate in water/acetonitrile/formic acid 2:3:0.005 v/v/v (eluent A) and 10 mM ammonium formate in isopropanol/acetonitrile/formic acid 9:1:0.01 v/v/v (eluent B). Linear gradient elution was as follows: 0– 2 min from 40 to 43% eluent B, 2–12 min from 50 to 54% eluent B, 12-18 min from 70 to 99% eluent B, and 18–20 min 40% eluent B.. The column temperature was set at 55 °C, and the flow rate was 0.4 mL/min. One μL of sample was loaded to MaXis plus high-resolution QTof mass spectrometer (Bruker, Bremen, Germany), Lipids were detected by electrospray ionization in positive (ESI+) and negative mode (ESI-).

Treatment ID:

TR002080

Treatment Summary:

The diluted lipid solution was separated using a Zorbax Eclipse Plus C18 column (1.8 μm, 50 × 2.1 mm) on an Acquity UPLC system (Waters, Manchester, UK). Mobile phases consisted of 10 mM ammonium formate in water/acetonitrile/formic acid 2:3:0.005 v/v/v (eluent A) and 10 mM ammonium formate in isopropanol/acetonitrile/formic acid 9:1:0.01 v/v/v (eluent B). Linear gradient elution was as follows: 0– 2 min from 40 to 43% eluent B, 2–12 min from 50 to 54% eluent B, 12-18 min from 70 to 99% eluent B, and 18–20 min 40% eluent B.. The column temperature was set at 55 °C, and the flow rate was 0.4 mL/min. One μL of sample was loaded to MaXis plus high-resolution QTof mass spectrometer (Bruker, Bremen, Germany), Lipids were detected by electrospray ionization in positive (ESI+) and negative mode (ESI-).