Summary of Study ST002186
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001393. The data can be accessed directly via it's Project DOI: 10.21228/M8NT4K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002186 |
Study Title | An early-life microbiota metabolite protects against obesity via intestinal PPAR-gamma |
Study Type | untargeted metabolomics analysis |
Study Summary | The mechanisms by which the early-life microbiota protects against environmental factors that promote childhood obesity remain largely unknown. Using a mouse model in which young mice are simultaneously exposed to antibiotics and a high-fat diet, we show that Lactobacillus species, predominant members of the small intestine microbiota, regulate intestinal epithelial cells (IECs) to limit diet-induced obesity during early-life. A Lactobacillus-derived metabolite, phenyllactic acid (PLA), protected against metabolic dysfunction caused by early-life exposure to antibiotics and a high-fat diet by increasing the abundance of peroxisome proliferator activated receptor gamma (PPAR-gamma) in the small intestine IECs. Therefore, PLA is a microbiota-derived metabolite that activates protective pathways in the small intestine epithelium to regulate fat absorption and prevent obesity during early life. |
Institute | Vanderbilt University |
Department | Chemistry |
Laboratory | Center for Innovative Technology |
Last Name | Codreanu |
First Name | Simona Gabriella |
Address | 1234 Stevenson Center Lane |
SIMONA.CODREANU@VANDERBILT.EDU | |
Phone | 6158758422 |
Submit Date | 2022-06-03 |
Num Groups | 4 |
Total Subjects | 20 |
Num Males | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-06-06 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002284 |
Treatment Summary: | Animals were fed either a 60% fat diet (HF) (OpenSource Diets, #D12492) or a 10% fat control diet (LF) (OpenSource Diets, #D12450J) for 5 weeks. Groups of LF or HF diet mice were also given low doses of penicillin (Sigma Aldrich #P1382)(LDP) (6.67 mg/L, (10)) in their drinking water throughout the experiment or clinical doses of penicillin (0.167 g/L, (35)) in their drinking water from days 0 – 5, 15 – 20, and 30 – 35. For long-term experiments, mice were fed a HF diet or given a HF diet and LDP for 5 weeks before being switched to either a LF or HF diet alone for an additional 5 weeks. |