Summary of Study ST002359

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001514. The data can be accessed directly via it's Project DOI: 10.21228/M80H6B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002359
Study Title	Effect of hypoxia and reoxygenation on the adult hooded seal brain lipidome
Study Summary	Brain samples from adult hooded seals (Cystophora cristata) were subjected to hypoxia and reoxygenation in vitro and lipid composition was compared.
Institute	University of Hamburg
Last Name	Martens
First Name	Gerrit Alexander
Address	Martin-Luther-King-Platz 3, 20146 Hamburg, Germany
Email	gerrit.alexander.martens@uni-hamburg.de
Phone	+49 40 42838-3934
Submit Date	2022-11-23
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	LC-MS
Release Date	2023-04-12
Release Version	1

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Treatment:

Treatment ID:	TR002460
Treatment Summary:	Samples in aCSF were adjusted to 34±0.5°C for at least 20 min. Hypoxia was introduced and maintained for 60 min after switching the gas supply to 95% N2 and 5% CO2, to mimic the diving brain. To simulate conditions when the seal surfaces after a dive, samples were exposed to hypoxia followed by 20 min return to normoxia. After treatment, hypoxia and reoxygenation samples were immediately frozen in liquid nitrogen. Samples that were kept under normoxia in aCSF for 80 min were used as controls. All samples were transferred to and stored at -80°C until later use.

Treatment ID:

TR002460

Treatment Summary:

Samples in aCSF were adjusted to 34±0.5°C for at least 20 min. Hypoxia was introduced and maintained for 60 min after switching the gas supply to 95% N2 and 5% CO2, to mimic the diving brain. To simulate conditions when the seal surfaces after a dive, samples were exposed to hypoxia followed by 20 min return to normoxia. After treatment, hypoxia and reoxygenation samples were immediately frozen in liquid nitrogen. Samples that were kept under normoxia in aCSF for 80 min were used as controls. All samples were transferred to and stored at -80°C until later use.