Summary of Study ST002537

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001633. The data can be accessed directly via it's Project DOI: 10.21228/M8MM8Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST002537
Study Title	Osmoprotectants play a major role in the Portulaca oleracea resistance to high levels of salinity stress - Insights from a metabolomics and proteomics integrated approach
Study Summary	Purslane is an invasive plant and is considered the eighth most common weed in the world. Because of that, its outdoor production in extensive areas faces several concerns. Kong & Zheng (2014) evaluated the potential of producing purslane in a hydroponic system, generating approximately 5.75 kg of fresh matter per m2 per month, which might yield 57.5 tons/hectare/year if cultivated in a bimestrial regime. The high productivity of purslane, when grown in controlled-environment agriculture, can open many opportunities for the purslane industry, even in the context of biosaline agriculture. Building up a robust multi-omics database on the response of purslane to salt stress and the subsequent study of it via an MOI analysis can create the basis for a future system biology approach to decode the genetics behind its resistance to salinity stress. The present study is a second step in building a robust database on the morpho-physiological and molecular responses of Portulaca oleracea L. to salinity stress and its subsequent use in attempting to decode the genetics behind its resistance to this abiotic stress. After reporting on the characterization of the morpho-physiological responses of young purslane plants to such stress using a robust salinization protocol, here we report a study on adult purslane plants through the characterization of the untargeted metabolome and proteome profiles on the leaves and roots of this halophyte species submitted to very high salinity stress, and the consequent use of single- and multi-omics analysis strategies to study it.
Institute	Embrapa Agroenergy
Last Name	Souza Júnior
First Name	Manoel Teixeira
Address	Parque Estacao Biologica final Asa Norte Brasília DF 70770-901 BR, PQEB, sn - Asa Norte, DF
Email	manoel.souza@embrapa.br
Phone	+55 (61) 3448-4246
Submit Date	2023-04-03
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2023-06-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Treatment:

Treatment ID:	TR002649
Treatment Summary:	The B1 accession of purslane (Portulaca oleracea L.) used in this study belongs to the Purslane Collection at Embrapa Agroenergia. Seeds underwent disinfection following the same procedure described in Belo Silva et al. (2022), which consisted of soaking in 2% sodium hypochlorite and Tween® 20 for five minutes, under slow agitation, and then washing with sterile water and drying on sterilized filter paper. After being seeded on a culture medium (MS 1/2 strength, Phytagel 0.2%, and pH 5.8) (Murashige and Skoog, 1962), it was kept for germination in a Growth chamber Conviron mod. Adaptis 1000TC (Controlled Environments Ltd, Winnipeg, Canada) at 150 μmol/m2/s of light and 30°C. After 13 days, seedlings were individually transferred to 200 ml plastic cups containing 100 g of sterilized substrate - clay soil, vermiculite, and a commercial substrate (Bioplant®), 2:1:1 (v:v:v) ratio – and transferred to another Conviron® growth chamber mod. PGW40 at 25±2°C, 500±20 μmol/m2/s of light, 65±5% air relative humidity, and photoperiod of 16/8 h (light/dark), and kept there until the end of the experiments. The plants were allowed to acclimatize for three days, and the salinity stress started three weeks after the end of the acclimatization period. The salinization experiment consisted of two salinity levels (0.0 and 2.0 g of NaCl / 100 g of the substrate), with 16 replicates (plants) in a completely randomized design, and the stress lasted 12 days. During the entire experiment, plants were at field capacity. To avoid the loss of Na+ or Cl-, no leakage of the saline solution was allowed to get out of the plastic cup, as described previously in Belo Silva et al. (2022). The water lost due to evapotranspiration was replaced with deionized water daily, and the electric conductivity at field capacity (ECfc) and water potential in the substrate solution was measured once - on the 8th day of stress - for all replicates.

Treatment ID:

TR002649

Treatment Summary:

The B1 accession of purslane (Portulaca oleracea L.) used in this study belongs to the Purslane Collection at Embrapa Agroenergia. Seeds underwent disinfection following the same procedure described in Belo Silva et al. (2022), which consisted of soaking in 2% sodium hypochlorite and Tween® 20 for five minutes, under slow agitation, and then washing with sterile water and drying on sterilized filter paper. After being seeded on a culture medium (MS 1/2 strength, Phytagel 0.2%, and pH 5.8) (Murashige and Skoog, 1962), it was kept for germination in a Growth chamber Conviron mod. Adaptis 1000TC (Controlled Environments Ltd, Winnipeg, Canada) at 150 μmol/m2/s of light and 30°C. After 13 days, seedlings were individually transferred to 200 ml plastic cups containing 100 g of sterilized substrate - clay soil, vermiculite, and a commercial substrate (Bioplant®), 2:1:1 (v:v:v) ratio – and transferred to another Conviron® growth chamber mod. PGW40 at 25±2°C, 500±20 μmol/m2/s of light, 65±5% air relative humidity, and photoperiod of 16/8 h (light/dark), and kept there until the end of the experiments. The plants were allowed to acclimatize for three days, and the salinity stress started three weeks after the end of the acclimatization period. The salinization experiment consisted of two salinity levels (0.0 and 2.0 g of NaCl / 100 g of the substrate), with 16 replicates (plants) in a completely randomized design, and the stress lasted 12 days. During the entire experiment, plants were at field capacity. To avoid the loss of Na+ or Cl-, no leakage of the saline solution was allowed to get out of the plastic cup, as described previously in Belo Silva et al. (2022). The water lost due to evapotranspiration was replaced with deionized water daily, and the electric conductivity at field capacity (ECfc) and water potential in the substrate solution was measured once - on the 8th day of stress - for all replicates.