Summary of project PR000130
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000130. The data can be accessed directly via it's Project DOI: 10.21228/M81P4M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000130 |
| Project DOI: | doi: 10.21228/M81P4M |
| Project Title: | High insulin combined with essential amino acids stimulates skeletal muscle mitochondrial protein synthesis while decreasing insulin sensitivity in healthy humans |
| Project Type: | Targeted metabolomics |
| Project Summary: | CONTEXT:Insulin and essential amino acids (EAAs) regulate skeletal muscle protein synthesis, yet their independent effects on mitochondrial protein synthesis (MiPS) and oxidative function remain to be clearly defined. OBJECTIVE:The purpose of this study was to determine the effects of high or low insulin with or without EAAs on MiPS. DESIGN:Thirty participants were randomized to 3 groups of 10 each with each participant studied twice. Study groups comprised (1) low and high insulin, (2) low insulin with and without EAAs, and (3) high insulin with and without EAAs. SETTING:The study was conducted in an in-patient clinical research unit. PARTICIPANTS:Eligible participants were 18 to 45 years old, had a body mass index of <25 kg/m(2), and were free of diseases and medications that might impair mitochondrial function. INTERVENTION:Low (? 6 ?U/mL) and high (? 40 ?U/mL) insulin levels were maintained by iv insulin infusion during a somatostatin clamp while maintaining euglycemia (4.7-5.2 mM) and replacing GH and glucagon. The EAA infusion was 5.4% NephrAmine. l-[ring-(13)C6]Phenylalanine was infused, and muscle needle biopsies were performed. MAIN OUTCOMES:Muscle MiPS, oxidative enzymes, and plasma amino acid metabolites were measured. RESULTS:MiPS and oxidative enzyme activities did not differ between low and high insulin (MiPS: 0.07 ± 0.009 vs 0.07 ± 0.006%/h, P = .86) or between EAAs and saline during low insulin (MiPS: 0.05 ± 0.01 vs 0.07 ± 0.01, P = .5). During high insulin, EAAs in comparison with saline increased MiPS (0.1 ± 0.01 vs 0.06 ± 0.01, P < .05) and cytochrome c oxidase activity (P < .05) but not citrate synthase (P = .27). EAA infusion decreased (P < .05) the glucose infusion rates needed to maintain euglycemia during low (? 40%) and high insulin (? 24%). CONCLUSION:EAAs increased MiPS and oxidative enzyme activity only with high insulin concentrations. |
| Institute: | Mayo Clinic |
| Department: | Endocrinology |
| Laboratory: | Dr. Sreekumaran Nair's lab |
| Last Name: | Nair |
| First Name: | Sreekumaran |
| Address: | Mayo Clinic, 200 First Street SW, Rochester, MN |
| Email: | Dasari.Surendra@mayo.edu |
| Funding Source: | 5T32DK007352, 5R01DK041973, UL1 TR000135, U24DK100469 |
| Project Comments: | 25222757 |
Summary of all studies in project PR000130
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST000149 | High Insulin Combined With Essential Amino Acids Stimulates Skeletal Muscle Mitochondrial Protein Synthesis While Decreasing Insulin Sensitivity in Healthy Humans | Homo sapiens | Mayo Clinic | MS | 2015-03-05 | 1 | 120 | Uploaded data (1.5M) |
