Summary of project PR000280

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000280. The data can be accessed directly via it's Project DOI: 10.21228/M8QK55 This work is supported by NIH grant, U2C- DK119886.


Project ID: PR000280
Project DOI:doi: 10.21228/M8QK55
Project Title:Application of a targeted metabolomic profiling for the delineation of mechanisms of autolytic systems in Streptococcus mutans cells under oxidative stresses
Project Summary:Streptococcus mutans is capable of withstanding a variety of stressors encountered in the oral cavity. The ability to efficiently and rapidly adjust to the changing environment is essential for its pathogenic lifestyle. Oxidative stress is one of the most important environmental variables affecting the pathogenic potential of S. mutans, as high oxygen concentrations disfavor growth of S. mutans and other oral bacteria. In order to fulfill its role as a major constituent of cariogenic oral biofilms and sustain virulence, S. mutans must overcome oxidative stress. Oxidation sensing and response is a complex yet highly-regulated process, particularly because it is cross-regulated with other metabolic pathways, stress responses and virulence physiology. In addition, we previously showed that exposure of S. mutans to oxygen strongly inhibits biofilm formation and alters cell surface biogenesis, possibly through AtlA, a major autolysin of the organism. More recently, we have also shown that S. mutans Cid/Lrg system, potentially mediating cell lysis/death in a programmed fashion, uniquely leads a global readjustment in central metabolism and virulence processes, particularly in coping with oxidative stress. To this end, a targeted metabolomics approach will be employed to understand how these autolytic systems influence the metabolism of S. mutans, ultimately leading to a more complete appreciation of how S. mutans survives and persists in hostile host microenvironment.
Institute:University of Florida
Department:College of Dentistry
Last Name:Ahn
First Name:Sang-Joon
Address:1395 Center Drive, Room D5-29 Gainesville FL 32610
Funding Source:NIH R03

Summary of all studies in project PR000280

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST000351 Determining the metabolic profile of wildtype, lrgAB, and atlA mutant Steptococcus mutans grown aerobically and anaerobically Streptococcus mutans University of Florida MS 2019-01-22 1 33 Uploaded data (2.7M)