Summary of project PR000613
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000613. The data can be accessed directly via it's Project DOI: 10.21228/M8F681 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000613 |
| Project DOI: | doi: 10.21228/M8F681 |
| Project Title: | Evidence that COG0325 proteins are involved in Pyridoxal 5'-phosphate (PLP) homeostasis |
| Project Summary: | Pyridoxal 5'-phosphate (PLP) is an essential cofactor for nearly 60 Escherichia coli enzymes but is a highly reactive molecule that is toxic in its free form. How PLP levels are regulated and how PLP is delivered to target enzymes are still open questions. The COG0325 protein family belongs to the fold-type III class of PLP enzymes and binds PLP but has no known biochemical activity although it occurs in all kingdoms of life. Various pleiotropic phenotypes of the E. coli COG0325 (yggS) mutant have been reported, some of which were reproduced and extended in this study. Comparative genomic, genetic and metabolic analyses suggest that these phenotypes reflect an imbalance in PLP homeostasis. The E. coli yggS mutant accumulates the PLP precursor pyridoxine 5'-phosphate (PNP) and is sensitive to an excess of pyridoxine but not of pyridoxal. The pyridoxine toxicity phenotype is complemented by the expression of eukaryotic yggS orthologs. It is also suppressed by the presence of amino acids, specifically isoleucine, threonine and leucine, suggesting the PLP-dependent enzyme transaminase B (IlvE) is affected. These genetic results lay a foundation for future biochemical studies of the role of COG0325 proteins in PLP homeostasis. |
| Institute: | University of Florida |
| Department: | Microbiology and Cell Science |
| Last Name: | de Crecy-Lagard |
| First Name: | Valerie |
| Address: | Gainesville, FL 32611 |
| Email: | vcrecy@ufl.edu |
| Phone: | 352-392-9416 |
Summary of all studies in project PR000613
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST000884 | Evidence that COG0325 proteins are involved in PLP homeostasis | Escherichia coli | University of California, Davis | MS* | 2017-10-14 | 1 | 183 | Uploaded data (1.2G)* |
