Summary of project PR000614

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000614. The data can be accessed directly via it's Project DOI: 10.21228/M8KD9Q This work is supported by NIH grant, U2C- DK119886.


Project ID: PR000614
Project DOI:doi: 10.21228/M8KD9Q
Project Title:Gut Microbiota Modulate Brain Insulin Sensitivity and Neurobehavior
Project Type:Metabolite profiling of cecal contents and brains of mice under diet-induced obesity (DIO) with and without antibiotic treatments.
Project Summary:Obesity and diabetes in humans are associated with increased rates of anxiety and depression. To understand the role of the gut microbiome and brain insulin resistance in these disorders, we evaluated behaviors and insulin action in brain of mice with diet-induced obesity (DIO) with and without antibiotic treatment. We find that DIO mice have behaviors reflective of increased anxiety and depression. This is associated with decreased insulin signaling and increased inflammation in multiple brain regions. Treatment with oral metronidazole or vancomycin decreases inflammation, improves insulin signaling in the brain and reduces signs of anxiety and depression. These effects are transferable to germ-free mice by fecal transplant of gut microbiota and are associated with changes in the levels of tryptophan, GABA, BDNF, amino acids and multiple acylcarnitines in the brain. Thus, changes in gut microbiota can control brain insulin signaling and metabolite levels, and this leads to altered neurobehaviors.
Institute:Broad Institute of MIT and Harvard
Department:Metabolomics Platform
Last Name:Avila-Pacheco
First Name:Julian
Address:415 Main Street, Rm 7175, Cambridge, MA, 02142, USA
Contributors:Marion Soto, Clémence Herzog, Julian Avila-Pacheco, Shiho Fujisaka, Kevin Bullock, Clary B. Clish, and C. Ronald Kahn

Summary of all studies in project PR000614

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST000885 Gut Microbiota Modulate Brain Insulin Sensitivity and Neurobehavior Mus musculus Broad Institute of MIT and Harvard MS 2017-10-30 1 71 Uploaded data (65.8M)*