Summary of project PR000629
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000629. The data can be accessed directly via it's Project DOI: 10.21228/M8C972 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000629 |
| Project DOI: | doi: 10.21228/M8C972 |
| Project Title: | Murine vitamin A deficiency results in a hypermetabolic state and alterations in bacterial community structure and metabolism |
| Project Summary: | Vitamin A deficiency (A-) is a significant public health problem. To better understand how vitamin A status influences gut microbiota and host metabolism, we systematically analyzed urine, cecum, serum, and liver samples from vitamin A sufficient (A+) and A- mice using 1H NMR-based metabolomics, quantitative (q)PCR, and 16S rRNA gene sequencing coupled with multivariate data analysis. The microbiota in the cecum of A- mice showed compositional as well as functional shifts compared to the microbiota from A+ mice. Targeted 1H NMR analyses revealed significant changes in microbial metabolite concentrations including higher butyrate and hippurate and decreased acetate and 4-hydroxyphenylacetate in A+ relative to A- mice. Bacterial butyrate-producing genes including butyryl-CoA:acetate CoA-transferase and butyrate kinase were significantly higher in bacteria from A+ versus bacteria from A- mice. A - mice had disturbances in multiple metabolic pathways including alterations in energy metabolism (hyperglycemia, glycogenesis, TCA cycle, and lipoprotein biosynthesis) and the A- host showed metabolites indicative of a hypermetabolic state (higher levels of amino acids and nucleic acids). A- mice had hyperglycemia, liver dysfunction, changes in bacterial metabolism, and altered gut microbial communities. Moreover, integrative analyses indicated a strong correlation between gut microbiota and host energy metabolism pathways in the liver. Vitamin A regulates the microbiota, bacterial metabolism and the effects of vitamin A on the microbiota results in alterations to host metabolism. |
| Institute: | Pennsylvania State University |
| Last Name: | Nichols |
| First Name: | Robert |
| Address: | 101 Life science building, University Park, State college, PA, 16803 |
| Email: | rgn5011@psu.edu |
| Phone: | 7247662694 |
Summary of all studies in project PR000629
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST000905 | Murine vitamin A deficiency results in a hypermetabolic state and alterations in bacterial community structure and metabolism. (Liver) | Mus musculus | Pennsylvania State University | NMR | 2018-02-07 | 1 | 12 | Uploaded data (24.9M) |
| ST000906 | Murine vitamin A deficiency results in a hypermetabolic state and alterations in bacterial community structure and metabolism. (Serum) | Mus musculus | Pennsylvania State University | NMR | 2018-02-07 | 1 | 12 | Uploaded data (24.9M) |
| ST000907 | Murine vitamin A deficiency results in a hypermetabolic state and alterations in bacterial community structure and metabolism.(Urine) | Mus musculus | Pennsylvania State University | NMR | 2018-02-07 | 1 | 12 | Uploaded data (24.9M) |
| ST000908 | Murine vitamin A deficiency results in a hypermetabolic state and alterations in bacterial community structure and metabolism. (Cecal contents) | Mus musculus | Pennsylvania State University | NMR | 2018-02-07 | 1 | 12 | Uploaded data (24.9M) |
