Summary of project PR000708
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000708. The data can be accessed directly via it's Project DOI: 10.21228/M8596T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000708 |
| Project DOI: | doi: 10.21228/M8596T |
| Project Title: | Metabolic approaches reveal the role of CAR in energy metabolism |
| Project Type: | Time Course |
| Project Summary: | The constitutive androstane receptor (CAR; NR1I3) contributes important regulatory roles in biotransformation, xenobiotic transport function, energy metabolism and lipid homeostasis. In this investigation, global serum and liver tissue metabolomes were assessed analytically in wild type and CAR-null transgenic mice using NMR, GC/MS and UPLC/MS-MS-based metabolomics. Significantly, CAR activation increased serum levels of fatty acids, lactate, ketone bodies and tricarboxylic acid cycle products, whereas levels of phosphatidylcholine, sphingomyelin, amino acids and liver glucose were decreased following short-term activation of CAR. Mechanistically, quantitative mRNA analysis demonstrated significantly decreased expression of key gluconeogenic pathways, and increased expression of glucose utilization pathways, changes likely resulting from down-regulation of the hepatic glucose sensor and bi-directional transporter, Glut2. Short-term CAR activation also resulted in enhanced fatty acid synthesis and impaired β-oxidation. In summary, CAR contributes an expansive role regulating energy metabolism, significantly impacting glucose, and monocarboxylic acid, as well as fatty acid metabolism and lipid homeostasis, through receptor-mediated regulation of several genes in multiple associated pathways. |
| Institute: | Pennsylvania State University |
| Laboratory: | Omiecinski Lab |
| Last Name: | Omiecinski |
| First Name: | Curt |
| Address: | 101 Life Sciences Building |
| Email: | cjo10@psu.edu, dmw178@psu.edu |
| Phone: | 8148651572 |
Summary of all studies in project PR000708
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001055 | Fatty acid profiling of liver tissue using GC-MS. | Mus musculus | Pennsylvania State University | MS | 2019-01-22 | 1 | 50 | Uploaded data (1.5G)* |
| ST001058 | Total serum global lipid profiling by UPLC-MS. | Mus musculus | Pennsylvania State University | MS | 2019-01-22 | 1 | 12 | Uploaded data (5.6M)* |
| ST001092 | Metabolic profiling by NMR analysis in liver aqueous extract samples | Mus musculus | Pennsylvania State University | NMR* | 2019-01-22 | 1 | 24 | Uploaded data (54M)* |
