Summary of project PR000807

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000807. The data can be accessed directly via it's Project DOI: 10.21228/M8CD73 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR000807
Project DOI:doi: 10.21228/M8CD73
Project Title:A comprehensive time-course metabolite profiling of the model cyanobacterium Synechocystis sp. PCC 6803 under diurnal light:dark cycles
Project Summary:Cyanobacteria are a model photoautotroph and a chassis for the sustainable production of fuels and chemicals. Yet, knowledge of photoautotrophic metabolism in the natural environment of day/night cycles is lacking yet has implications for improved yield from plants, algae, and cyanobacteria. Here, a thorough approach to characterizing diverse metabolites—including carbohydrates, lipids, amino acids, pigments, co-factors, nucleic acids and polysaccharides—in the model cyanobacterium Synechocystis sp. PCC 6803 (S. 6803) under sinusoidal diurnal light-dark cycles was developed and applied. A custom photobioreactor and novel multi-platform mass spectrometry workflow enabled metabolite profiling every 30-120 minutes across a 24-hour diurnal sinusoidal LD (“sinLD”) cycle peaking at 1,600 mol photons m 2 s-1. We report widespread oscillations across the sinLD cycle with 90%, 94%, and 40% of the identified polar/semi-polar, non-polar, and polymeric metabolites displaying statistically significant oscillations, respectively. Microbial growth displayed distinct lag, biomass accumulation, and cell division phases of growth. During the lag phase, amino acids (AA) and nucleic acids (NA) accumulated to high levels per cell followed by decreased levels during the biomass accumulation phase, presumably due to protein and DNA synthesis. Insoluble carbohydrates displayed sharp oscillations per cell at the day-to-night transition. Potential bottlenecks in central carbon metabolism are highlighted. Together, this report provides a comprehensive view of photosynthetic metabolite behavior with high temporal resolution, offering insight into the impact of growth synchronization to light cycles via circadian rhythms. Incorporation into computational modeling and metabolic engineering efforts promises to improve industrially-relevant strain design.
Institute:Colorado State University
Department:Chemical and Biological Engineering
Last Name:Peebles
First Name:Christie
Address:700 Meridian Ave, Fort Collins, CO 80523 USA
Email:wernerajz@gmail.com
Phone:2699981811

Summary of all studies in project PR000807

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
Release
Date
VersionSamplesDownload
(* : Contains raw data)
ST001195 Non-targeted GC-MS Analysis of Polar Soluble Fraction (part-I) Synechocystis sp. PCC6803 Colorado State University MS* 2019-07-17 1 80 Uploaded data (2.3G)*
ST001196 Non-targeted GC-MS Analysis of Insoluble Metabolites (part-II) Synechocystis sp. PCC6803 Colorado State University MS* 2019-07-17 1 84 Uploaded data (1.3G)*
ST001197 GC-MS Analysis of Insoluble/Polymeric Amino Acids (part-III) Synechocystis sp. PCC6803 Colorado State University MS 2019-07-17 1 85 Uploaded data (656.8K)*
ST001198 Targeted LC-MS/MS Analysis of Soluble Metabolites in the MeOH:H2O Phase (part-IV) Synechocystis sp. PCC6803 Colorado State University MS 2019-07-17 1 85 Uploaded data (26.2M)*
ST001199 Non-targeted LC-MS Analysis of Soluble Metabolites in the Non-Polar MTBE Phase (part-V) Synechocystis sp. PCC6803 Colorado State University MS* 2019-07-17 1 83 Uploaded data (8.3G)*
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