Summary of project PR000850

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000850. The data can be accessed directly via it's Project DOI: 10.21228/M8T98G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR000850
Project DOI:doi: 10.21228/M8T98G
Project Title:Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy
Project Type:Metabolomics using Mass Spectrometry
Project Summary:Metabolic profiling of cancer cells can play an important role in revealing the molecular bases of cancer development and progression. In this work, gas chromatography coupled with mass spectrometry (GC-MS) was employed for the determination of signatures found in ER+/PR+ breast cancer cells derived from MCF-7 using different extraction solvents including: A, formic acid in water; B, ammonium hydroxide in water; C, ethyl acetate; D, methanol: water (1:1, v/v); and E, acetonitrile: water (1:1, v/v). The greatest extraction rate and diversity of metabolites occurs with extraction solvents A and E. Extraction solvent D showed moderate extraction efficiency, whereas extraction solvent B and C showed inferior metabolite diversity. Metabolite set enrichment analysis results showed energy production pathways to be key in MCF-7 cell lines. This study showed that mass spectrometry could identify key metabolites associated with cancers. The highest enriched pathways were related to energy production as well as Warburg effect pathways, which may shed light on how energy metabolism has been hijacked to encourage tumour progression and eventually metastasis in breast cancer.
Institute:University of Sharjah
Department:Sharjah Institute for Medical Research
Laboratory:Mohammad Semreen
Last Name:Hamoudi
First Name:Rifat
Address:College of Medicine, University of Sharjah
Email:rhamoudi@sharjah.ac.ae
Phone:567154756
Funding Source:Al-Jalila Foundation (Grant No: AJF201741), Breast Cancer Now (Grant No: 2014MaySP323) and University of Sharjah
Project Comments:Multidisciplinary based on mass spectrometry and bioinformatics

Summary of all studies in project PR000850

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ST001265 Comparative metabolomics of MCF-7 breast cancer cells using different extraction solvents assessed by mass spectroscopy Homo sapiens Sharjah Institute for Medical Research MS 2019-10-11 1 5 Uploaded data (75M)*
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