Summary of project PR000856
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000856. The data can be accessed directly via it's Project DOI: 10.21228/M81T3M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000856 |
| Project DOI: | doi: 10.21228/M81T3M |
| Project Title: | Growth cone Membrane lipidomics and Growth cone particulate at different developmental stages in C57BL/6 mice |
| Project Type: | untargeted LC-MS/MS lipidomics |
| Project Summary: | We performed high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP). These fractions were generated through established differential centrifugation techniques and lipid extraction. Developmental time points were chosen based upon our understanding of brain development in the murine model. In mice cortex, E18 is characterized by extension or elongation of axons toward targets, while around P0-P3 is when axons start innervating targets and branching occurs. At P6-P7 is when target innervation is generally complete and a subpopulation of cortical neurons undergo developmental cell death. By P9 growth cones have mostly disappeared. |
| Institute: | University of Miami |
| Department: | Ophthalmology, Bascom Palmer Eye Institute |
| Laboratory: | Sanjoy K. Bhattacharya |
| Last Name: | Bhattacharya |
| First Name: | Sanjoy |
| Address: | 1638 NW 10th Avenue Miami, FL 33136 |
| Email: | SBhattacharya@med.miami.edu |
| Phone: | 305-482-4103 |
| Contributors: | Jennifer Arcuri |
Summary of all studies in project PR000856
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001272 | Growth cone memebrane and growth cone particulate lipidomics | Mus musculus | University of Miami | MS | 2020-01-03 | 1 | 120 | Uploaded data (1.2G)* |
