Summary of project PR000871
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000871. The data can be accessed directly via it's Project DOI: 10.21228/M83M5X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR000871 |
| Project DOI: | doi: 10.21228/M83M5X |
| Project Title: | Regulated accumulation of desmosterol integrates macrophage lipid metabolism and inflammatory responses |
| Project Summary: | Inflammation and macrophage foam cells are characteristic features of atherosclerotic lesions, but the mechanisms linking cholesterol accumulation to inflammation and LXR-dependent response pathways are poorly understood. To investigate this relationship, we utilized lipidomic and transcriptomic methods to evaluate the effect of diet and LDL receptor genotype on macrophage foam cell formation within the peritoneal cavities of mice. Foam cell formation was associated with significant changes in hundreds of lipid species and unexpected suppression, rather than activation, of inflammatory gene expression. We provide evidence that regulated accumulation of desmosterol underlies many of the homeostatic responses observed in macrophage foam cells, including activation of LXR target genes, inhibition of SREBP target genes, selective reprogramming of fatty acid metabolism and suppression of inflammatory response genes. These observations suggest that macrophage activation in atherosclerotic lesions results from extrinsic, pro-inflammatory signals generated within the artery wall that suppress homeostatic and anti-inflammatory functions of desmosterol. |
| Institute: | University of California, San Diego |
| Department: | Bioengineering |
| Last Name: | Fahy |
| First Name: | Eoin |
| Address: | 9500 Gilman, La Jolla, CA, 92093, USA |
| Email: | efahy@ucsd.edu |
| Phone: | 858-534-4076 |
Summary of all studies in project PR000871
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001289 | Regulated accumulation of desmosterol integrates macrophage lipid metabolism and inflammatory responses | Mus musculus | LIPID MAPS | MS | 2020-01-22 | 1 | 36 | Not available |
