Summary of project PR000944

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000944. The data can be accessed directly via it's Project DOI: 10.21228/M8P41V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR000944
Project DOI:doi: 10.21228/M8P41V
Project Title:Fast and sensitive flow-injection mass spectrometry metabolomics by analyzing sample specific ion distributions
Project Summary:Mass spectrometry based metabolomics is a widely used approach in biotechnology and biomedical research. However, current methods coupling mass spectrometry with chromatography are time-consuming and not suitable for high-throughput analysis of thousands of samples. An alternative approach is flow-injection mass spectrometry (FI-MS) in which samples are directly injected to the ionization source. Here, we show that the sensitivity of Orbitrap FI-MS metabolomics methods is limited by ion competition effect in the detection system. We describe an approach for overcoming this effect by analyzing the distribution of ion m/z values and computationally determining a series of optimal scan ranges. This enables reproducible detection of ~9,000 and ~10,000 m/z features in metabolomics and lipidomics analysis of serum samples, respectively, with a sample scan time of ~15 seconds and duty time of ~30 seconds; a ~50% increase versus current spectral-stitching FI-MS. This approach facilitates high-throughput metabolomics for a variety of applications, including biomarker discovery and functional genomics screens.
Institute:Technion – Israel Institute of Technology
Laboratory:Prof. Tomer Shlomi Lab
Last Name:Shlomi
First Name:Tomer
Address:Technion
Email:tomersh@cs.technion.ac.il
Phone:+972-77-8871497

Summary of all studies in project PR000944

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
Release
Date
VersionSamplesDownload
(* : Contains raw data)
ST001380 Fast and sensitive flow-injection mass spectrometry metabolomics by analyzing sample specific ion distributions Homo sapiens Technion – Israel Institute of Technology MS* 2020-05-22 1 12 Uploaded data (6.2G)*
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