Summary of project PR000946

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000946. The data can be accessed directly via it's Project DOI: 10.21228/M8DM63 This work is supported by NIH grant, U2C- DK119886.


Project ID: PR000946
Project DOI:doi: 10.21228/M8DM63
Project Title:Distinct metabolic states of a cell guide alternate fates of mutational buffering through altered proteostasis.
Project Summary:Changes in metabolism can alter the cellular milieu; can this also change intracellular proteostasis? Since proteostasis can modulate mutational buffering, if change in metabolism has the ability to change proteostasis, arguably, it should also alter mutational buffering. Building on this, we find that altered cellular metabolic states in E. coli buffer distinct mutations. Buffered-mutants had folding problems in vivo and were differently chaperoned in different metabolic states. Notably, this assistance was dependent upon the metabolites and not on the increase in canonical chaperone machineries. Additionally, we were able to reconstitute the folding assistance afforded by metabolites in vitro and propose that changes in metabolite concentrations have the potential to alter proteostasis. Collectively, we unravel that the metabolite pools are bona fide members of proteostasis and aid in mutational buffering. Given the plasticity in cellular metabolism, we posit that metabolic alterations may play an important role in the positive or negative regulation of proteostasis.
Institute:CSIR-National Chemical Laboratory
Department:Biochemical Sciences Division
Last Name:Shanmugam
First Name:Dhanasekaran
Address:Dr. Homi Bhabha Road, Pune, maharashtra, 411008, India

Summary of all studies in project PR000946

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST001382 Distinct metabolic states of a cell guide alternate fates of mutational buffering through altered proteostasis Escherichia coli CSIR-National Chemical Laboratory MS 2020-06-01 1 23 Not available