Summary of project PR000956

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000956. The data can be accessed directly via it's Project DOI: 10.21228/M84386 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR000956
Project DOI:doi: 10.21228/M84386
Project Title:Sea-ice diatom compatible solute shifts
Project Type:Marine Metabolomics
Project Summary:Sea-ice algae provide an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2,3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4 fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, reached the highest intracellular compatible solute concentrations of ~ 85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice.
Institute:University of Washington
Department:School of Oceanography
Laboratory:Ingalls Lab
Last Name:Dawson
First Name:Hannah
Address:1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA 98195
Email:hmdawson@uw.edu
Phone:2062216750
Funding Source:Booth Foundation, NSF, UW Graduate Top Scholar Award, Gordon and Betty Moore Foundation
Publications:Dawson et al., Elementa

Summary of all studies in project PR000956

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
Release
Date
VersionSamplesDownload
(* : Contains raw data)
ST001393 Sea-ice diatom compatible solute shifts Nitzschia lecointei University of Washington MS 2020-09-29 1 20 Not available
  logo