Summary of project PR001010
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001010. The data can be accessed directly via it's Project DOI: 10.21228/M84T3J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001010 |
| Project DOI: | doi: 10.21228/M84T3J |
| Project Title: | Global metabolomics of IFNy cued neurogenic NSCs seeded on hydrogel |
| Project Summary: | Neural stem cells (NSCs) provide a strategy to replace damaged neurons following traumatic central nervous system injuries. A major hurdle to translation of this therapy is that direct application of NSCs to CNS injury does not support sufficient neurogenesis due to lack of proper cues. To provide prolonged spatial cues to NSCs IFN-γ was immobilized to biomimetic hydrogel substrate to supply physical and biochemical signals to instruct the encapsulated NSCs to be neurogenic. However, the immobilization of factors, including IFN-γ, versus soluble delivery of the same factor, has been incompletely characterized especially with respect to activation of signaling and metabolism in cells over longer time points. In this study, protein and metabolite changes in NSCs induced by immobilized versus soluble IFN-γ at 7 days were evaluated. Soluble IFN-γ, refreshed daily over 7 days, elicited stronger responses in NSCs compared to immobilized IFN-γ indicating that immobilization may not sustain signaling or has altered ligand/receptor interaction and integrity. However, both IFN-γ delivery types supported increased βIII tubulin expression in parallel with canonical and non-canonical receptor-signaling compared to no IFN-γ. Global metabolomics and pathway analysis revealed that soluble and immobilized IFN-γ altered metabolic pathway activities including energy, lipid and amino acid synthesis, with soluble IFN-γ having the greatest metabolic impact overall. |
| Institute: | University of Akron |
| Department: | Chemistry |
| Laboratory: | Shriver lab |
| Last Name: | Baumann |
| First Name: | Hannah |
| Address: | 190 E. Buchtel Common, Akron, OH, 44325, USA |
| Email: | hjb17@zips.uakron.edu |
| Phone: | 4196100269 |
| Funding Source: | NIH |
| Publications: | Metabolomic and Signaling Programs Induced by Immobilized versus Soluble IFN γ in Neural Stem Cells |
Summary of all studies in project PR001010
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001492 | Global metabolomics of IFNy cued neurogenic NSCs seeded on hydrogel | Rattus norvegicus | University of Akron | MS* | 2020-10-13 | 1 | 12 | Uploaded data (1G)* |
