Summary of project PR001127
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001127 |
| Project DOI: | doi: 10.21228/M81D6M |
| Project Title: | Redox metabolism measurement in mammalian cells and tissues by quantitative LC/MS method (part I) |
| Project Summary: | This study aimed to optimize the detection of several key redox-reactive metabolites from mammalian cells and tissues. We explored three different chromatographic methods and optimized sample preparation, extraction buffer and conditions as well as mass spectrometry detection parameters. The established method was tested and validated using biologically relevant ROS-inducing conditions. This study can be a valuable resource for the metabolomics community. |
| Institute: | Boston Childrens Hospital |
| Department: | Pathology |
| Laboratory: | Naama Kanarek |
| Last Name: | Petrova |
| First Name: | Boryana |
| Address: | 300 Longwood Av, Boston, MA, 2115, USA |
| Email: | boryana.petrova@childrens.harvard.edu |
| Phone: | 6173557433 |
Summary of all studies in project PR001127
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST001759 | Application of the redox metabolite detection method for mouse liver | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 21 | Uploaded data (1.6G)* |
| ST001760 | Application of the redox metabolite detection method for mouse kidney | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 22 | Uploaded data (1.6G)* |
| ST001761 | Application of the redox metabolite detection method for mouse biofluids | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 27 | Uploaded data (2G)* |
| ST001762 | Application of the redox metabolite detection method for mouse kidney (part II) | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 22 | Uploaded data (1.9G)* |
| ST001763 | Application of the redox metabolite detection method for mouse liver (part II) | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 22 | Uploaded data (1.9G)* |
| ST001764 | Application of the redox metabolite detection method for profiling redox state following pharmacologic perturbation with methotrexate | Homo sapiens | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 40 | Uploaded data (3.6G)* |
| ST001765 | Optimization of redox metabolite detection in mammalian cells (part I) | Homo sapiens | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 35 | Uploaded data (2.7G)* |
| ST001766 | Application of the redox metabolite detection method for mammalian tissues (part I) | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 16 | Uploaded data (2.4G)* |
| ST001767 | Application of the redox metabolite detection method for mammalian tissues (part II) | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 16 | Uploaded data (1.6G)* |
| ST001768 | Application of the redox metabolite detection method for mammalian tissues (part III) | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 14 | Uploaded data (1.2G)* |
| ST001769 | Application of the redox metabolite detection method for profiling redox state following pharmacologic perturbations of redox balance in cells (part I) | Homo sapiens | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 22 | Uploaded data (1.9G)* |
| ST001770 | Application of the redox metabolite detection method for profiling redox state following pharmacologic perturbations of redox balance in cells (part II) | Homo sapiens | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 29 | Uploaded data (1.8G)* |
| ST001771 | Application of the redox metabolite detection method for profiling redox state following pharmacologic perturbations of redox balance in cells (part III) | Homo sapiens | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 30 | Uploaded data (1.9G)* |
| ST001772 | Optimization of redox metabolite detection in mammalian cells (part II) | Homo sapiens | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 37 | Uploaded data (2.3G)* |
| ST001773 | Application of the redox metabolite detection method for mouse biofluids (part II) | Mus musculus | Boston Children's Hospital, Harvard Medical School | MS | 2021-05-17 | 1 | 24 | Uploaded data (1.9G)* |
