Summary of project PR001217

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001217. The data can be accessed directly via it's Project DOI: 10.21228/M8D693 This work is supported by NIH grant, U2C- DK119886.


Project ID: PR001217
Project DOI:doi: 10.21228/M8D693
Project Title:Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach.
Project Type:Untargeted Metabolomics
Project Summary:Entomopathogenic fungi have been successfully used to control agricultural pests. They infect insects by coming into direct contact with their cuticle or when feeding on contaminated leaves or fruits. After contact with the insect, the entomopathogenic fungus penetrates its body cavity, where it grows and colonizes it from within, causing its death The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. Despite recent developments and growing efforts to better understand fungal metabolism and metabolites, much remains unknown. Metabolomics therefore represents an important field for evaluating the metabolites produced or modified by an organism or its relationship with the environment. In the present study, we aim to use untargeted metabolomics with gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations caused by the co-cultivation of these strains and to correlate the metabolites produced by this consortium with the increased mortality in D. fovealis observed previously.
Institute:Universidade Federal do Paraná
Department:Patologia Básica
Laboratory:Laboratório de Microbiologia e Biologia Molecular
Last Name:Stuart
First Name:Andressa
Address:Av. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, Curitiba, Paraná, Brasil
Project Comments:Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The metabolomic analysis were performed at the Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). Three reads of every biological replicate (five per treatment) were performed, generating fifteen readings for each treatment. Pools of metabolites from each group were created as a quality control.
Contributors:Jason Lee Furuie, Thais Regiani Cataldi, Rodrigo Makowiecky Stuart, Maria Aparecida Cassilha Zawadneak, Carlos Alberto Labate, Ida Chapaval Pimentel

Summary of all studies in project PR001217

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST001927 Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach. Beauveria bassiana Universidade Federal do Paraná MS* 2022-10-03 1 15 Uploaded data (4.3G)*