Summary of project PR001293
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001293. The data can be accessed directly via it's Project DOI: 10.21228/M8KH6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001293 |
| Project DOI: | doi: 10.21228/M8KH6M |
| Project Title: | A Taguchi Design of Experiments Approach for Untargeted Metabolomics Sample Preparation Optimization |
| Project Type: | Design of Experiments - Sample preparation optimization |
| Project Summary: | Metabolomics commonly uses analytical techniques such as nuclear magnetic resonance (NMR) and liquid chromatography coupled to mass spectrometry (LC-MS) to quantify and identify metabolites associated with biological variation. Metabolome coverage from non-targeted LC-MS studies relies heavily on the pre-analytical protocols (e.g., homogenization and extraction) used. Chosen protocols impact which metabolites are successfully measured, which in turn impacts biological conclusions. Different homogenization and extraction methods produce significant variability in metabolome coverage, sample reproducibility, and extraction efficiency. Herein we describe an efficient Taguchi method design of experiments (DOE) approach to optimize the extraction solvent and volume, extraction time, and LC reconstitution solvent for a sequential non-polar and polar Caenorhabditis elegans extraction. DOE is rarely used in metabolomics yet provides a systematic approach for optimizing sample preparation while simultaneously decreasing the number of experiments required to obtain high-quality data. |
| Institute: | University of Georgia |
| Department: | Chemistry, Biochemistry, Complex Carbohydrate Research Center |
| Laboratory: | Edison Lab |
| Last Name: | Garcia |
| First Name: | Brianna |
| Address: | 315 Riverbend Road, Athens, GA, 30602, USA |
| Email: | brianna.garcia@uga.edu |
| Phone: | 6269059945 |
| Funding Source: | NIH Metabolomics Common Fund |
| Contributors: | Goncalo J. Gouveia, Amanda O. Shaver, I. Jonathan Amster, Arthur S. Edison, Franklin E. Leach III |
Summary of all studies in project PR001293
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002046 | A Taguchi Design of Experiments Approach for Untargeted Metabolomics Sample Preparation Optimization | Caenorhabditis elegans | University of Georgia | MS* | 2022-12-20 | 1 | 120 | Uploaded data (24G)* |
