Summary of project PR001337

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001337. The data can be accessed directly via it's Project DOI: 10.21228/M8WM52 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR001337
Project DOI:doi: 10.21228/M8WM52
Project Title:Metabolomics dataset of optogenetic axon regenerative mouse model post optic nerve crush
Project Summary:This metabolite dataset was collected from bacterial channel rhodopsin expressing transgenic mouse models subjected to optic nerve crush (ONC) with subsequent stimulation with light (promoting regeneration) or non-stimulation (lacking axon regeneration). ONC retains retinal ganglion cells within the retina, while degenerating axons. In transgenic bacterial channel rhodopsin expressing cells, light stimulation promotes regeneration. Genetically matched wild-type uninjured optic nerves, or control transgenic mice, were also analyzed. Metabolites were carefully extracted from finely minced optic nerve tissue using a solvent system (initial separation using 1:1 Methanol and H2O and second extraction using 8:1:1 of Acetonitrile:Acetone:Methanol). Untargeted liquid chromatography-mass spectrometry (LC-MS/MS) profiling was performed using fractionation on a Vanquish Horizon Binary UHPLC. Subsequent analyses were performed on an inline coupled Q-Exactive Orbitrap instrument. Metabolites were identified using Compound DiscovererTM software. Statistical analysis was performed using MetaboAnalyst 5.0.
Institute:University of Miami
Department:Ophthalmology
Last Name:Bhattacharya
First Name:Sanjoy K.
Address:1638 NW 10th Avenue, Room 706-A, Miami, FL 33136
Email:sbhattacharya@med.miami.edu
Phone:(305) 482-4103

Summary of all studies in project PR001337

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
Release
Date
VersionSamplesDownload
(* : Contains raw data)
ST002111 Metabolomics dataset of optogenetic axon regenerative mouse model post optic nerve crush Mus musculus University of Miami MS 2022-04-04 1 66 Uploaded data (10.9G)*
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