Summary of project PR001531

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001531. The data can be accessed directly via it's Project DOI: 10.21228/M8ST4T This work is supported by NIH grant, U2C- DK119886.


Project ID: PR001531
Project DOI:doi: 10.21228/M8ST4T
Project Title:Ruegeria pomeroyi transporter mutant substrate drawdown
Project Summary:The goal of this project was to identify bacterial transporters responsible for uptake of environmentally relevant marine metabolites. We used the model marine heterotrophic bacterium Ruegeria pomeroyi DSS-3, for which an arrayed library of single gene knockout mutants has been generated by selecting isolated from a barcoded transposon mutant library (BasSeq). Knockout mutants of putative transporters were grown on minimal medium with a single substrate as sole carbon source. Mutant defect was assessed by comparing the substrate drawdown of isolated mutants to drawdown by a pooled mutant library (BarSeq), a proxy for wildtype fitness.
Institute:University of Georgia
Laboratory:Moran Lab, Edison Lab
Last Name:Uchimiya
First Name:Mario
Address:315 Riverbend Rd, Athens, GA, 30602, USA
Phone:‭(706) 542-8387‬
Funding Source:Simons Foundation (542391), NSF (OCE-2019589)
Contributors:William F. Schroer

Summary of all studies in project PR001531

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST002381 Ruegeria pomeroyi transporter mutant substrate drawdown Ruegeria pomeroyi University of Georgia NMR 2022-12-27 1 316 Uploaded data (3.2G)*