Summary of project PR001552
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001552. The data can be accessed directly via it's Project DOI: 10.21228/M8342Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001552 |
| Project DOI: | doi: 10.21228/M8342Z |
| Project Title: | Metabolic effects of the protein kinase R |
| Project Type: | Untargeted LCMS metabolomics |
| Project Summary: | Spleen-derived macrophage from WT or Eif2ak2-/- (gene encoding PKR protein kinase) mice are treated with a synthetic RNA mimetic (polyinosinic:polycytidylic acid) to activate the kinase and metabolites were collected for analysis. The data identified 325 putative metabolites in the cell extracts, with a large number of significant differences between the Eif2ak2- /- and WT sample groups. Metabolite levels are predominantly suppressed in the WT compared to the Eif2ak2-/- cells, with depletion of specific metabolites in amino acid, carbohydrate, lipid and nucleotide pathways, while several amino acid metabolites were significantly elevated in the WT cells compared to the Eif2ak2-/-. The changes appear to delineate a pseudo-starvation response in the WT cells. Phosphate energy metabolism is altered with decreased creatine and phosphocreatine and a compensatory increase in phosphorylated guanidinoacetate in the WT compared to the Eif2ak2- /- cells. There appears to be a constraint in glycolysis in the WT cells, most clearly in the pentose phosphate pathway. |
| Institute: | Hudson Institute of Medical Research |
| Department: | CIIID |
| Laboratory: | Molecular Immunology |
| Last Name: | Sadler |
| First Name: | Anthony |
| Address: | 27-31 Wright St, Clayton, VIC 3168, Australia |
| Email: | Anthony.sadler@hudson.org.au |
| Phone: | +61 4 85722722 |
| Funding Source: | NHMRC grants (1143839, 1043398) and a philanthropic Perpetual Trusties grant (CF07/2408) and a philanthropic Perpetual IMPACT grant. |
| Publications: | Suppression of the nucleic acid precursor ribose 5-phosphate by RNA-mediated antiviral immunity |
| Contributors: | Pushpack Bhattacharjee, Die Wang, Dovile Anderson, Joshua N. Buckler, Eveline de Geus, Feng Alex Yan, Galina Polekhina, Ralf Schittenhelm, Darren J. Creek, Lawrence D. Harris, Anthony J. Sadler |
Summary of all studies in project PR001552
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST002412 | Metabolic effects of the protein kinase R | Mus musculus | Hudson Institute of Medical Research | MS | 2023-01-04 | 1 | 19 | Uploaded data (1.7G)* |
