Summary of project PR001627

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001627. The data can be accessed directly via it's Project DOI: 10.21228/M8D434 This work is supported by NIH grant, U2C- DK119886.


Project ID: PR001627
Project DOI:doi: 10.21228/M8D434
Project Title:Pancreatic tumors activate arginine biosynthesis to adapt to myeloid-driven amino acid stress
Project Summary:Nutrient stress in the tumor microenvironment requires cancer cells to adopt adaptive metabolic programs to maintain survival and proliferation. Therefore, knowledge of microenvironmental nutrient levels and how cancer cells cope with such nutrition is critical to understand the metabolism underpinning cancer cell biology. Previously, we performed quantitative metabolomics of the interstitial fluid (the local perfusate) of murine pancreatic ductal adenocarcinoma (PDAC) tumors to comprehensively characterize nutrient availability in the microenvironment of these tumors (Sullivan et al., 2019a). Here, we develop Tumor Interstitial Fluid Medium (TIFM), a cell culture medium that contains nutrient levels representative of the PDAC microenvironment, enabling study of PDAC metabolism under physiological nutrition. We show that PDAC cells cultured in TIFM, compared to standard laboratory models, adopt a cellular state more similar to PDAC cells in tumors. Further, using the TIFM model we identified arginine biosynthesis as a metabolic adaptation PDAC cells engage to cope with microenvironmental arginine starvation driven by myeloid cells in PDAC tumors. Altogether, these data show that nutrient availability in tumors is an important determinant of cancer cell metabolism and behavior, and cell culture models that incorporate physiological nutrient availability have improved fidelity and enable the discovery of novel cancer metabolic phenotypes.
Institute:University of Chicago
Last Name:Apiz Saab
First Name:Juan
Address:929 E. 57th St.

Summary of all studies in project PR001627

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST002526 Stable isotope tracing of 15N2-glutamine in orthotopic pancreatic ductal adenocarcinoma tumor bearing mice and non tumor-bearing controls Mus musculus University of Chicago MS 2023-04-13 1 28 Uploaded data (1.4G)*
ST002527 HPLC-MS-MS analysis amino acid levels in PDAC IF samples upon arginase inhibition Mus musculus University of Chicago MS 2023-04-13 1 21 Uploaded data (1.3G)*
ST002528 Concentrations of amino acids in interstitial fluid and whole tumor samples of a murine PDAC orthotopic tumor model, measured by LC-MS Mus musculus University of Chicago MS 2023-04-13 1 7 Uploaded data (413M)*
ST002529 In vivo 15N2-glutamine tracing by jugular vein infusion in PDAC-tumor bearing Lyz2-Arg1 and control mice Mus musculus University of Chicago MS 2023-04-13 1 55 Uploaded data (7.8G)*
ST002530 LCMS analysis of amino acid levels in PDAC interstitial fluid samples upon ass1 cancer cell knock out Mus musculus University of Chicago MS 2023-04-13 1 22 Uploaded data (1.3G)*
ST002531 Cellular consumption/release of polar metabolites by mPDAC cells cultured in different culture media conditions Mus musculus University of Chicago MS 2023-04-13 1 24 Uploaded data (1.4G)*